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Outline and Activity

Office of Support for Platforms for Advanced Technologies and Research Resources
Japan Society for the Promotion of Science
Database of Grants-in-Aid for Scientific Research

Support for Physiological Analysis

Head of the Group
Kazutaka Ikeda (Tokyo Metropolitan Institute of Medical Science)

Activity

Our support includes:

  1. Behavioral analysis of a comprehensive behavioral analysis, in vivo calcium imaging experiment, maintaining the mice under SPF conditions and breeding mice.
  2. Pharmacological analysis support for investigating behaviors on genetically modified mice and mice that are administered with drugs such as addictive drugs, and analyses of associations between the genetic polymorphisms and the sensitivity to controlled substances (clinical data).
  3. Analyze the determined and operated cell function with the cutting-edge optical technology.
  4. Analyze multidimensional physiological function using multifunctional and integrated electrodes.

Characteristics

1. Support for behavioral analysis

We conduct the following support at Center for Medical Science, Fujita Health University (Tsuyoshi Miyakawa) or Organization for Promotion of Research Administration Center for Promotion of Research, University of Toyama (Keizo Takao).

  1. Analyze a comprehensive behavior of genetically modified mice or drug-administered mice. (Fujita Health University and University of Toyama)
  2. If we determine some kind of phenotypes in the comprehensive behavioral analysis:
    We will conduct the additional behavioral experiment that is not included in the comprehensive behavioral analysis. (Fujita Health University and University of Toyama), and conduct additional in vivo Calcium imaging experiment of the mice exercising the target action. (Fujita Health University)
    We are capable to simultaneously record the activity of the several nerve cells in mice in free action using the apparatus from Inscopix, Inc.
  3. If an applicant cannot prepare mice for analysis, we maintain the mice under SPF conditions, and breeding mice for the analysis. (In cooperation with National Museum of Emerging Science and Innovation)

The Application Procedure (Japanese Only)

2. Support for pharmacological analysis

We conduct the following support at Tokyo Metropolitan Institute of Medical Science (Kazutaka Ikeda) or Toyama University (Atsumi Nitta).

  1. Analyze the associations between the genes that the applicants are interested in and sensitivity to controlled substances, by using the clinical data related to sensitivity to controlled substances, genome DNA, and databases of associations between genetic polymorphisms and sensitivity to controlled substances (Kazutaka Ikeda).
  2. Test locomotor activity of applicant's genetically modified animals before and after the administration with various controlled substances (or new synthetic compound) (Kazutaka Ikeda).
  3. When applicant's genetically modified animals show characteristic locomotor activities in the above tests or reportedly showed any characteristic reactions to controlled substances, conduct special analyses, such as measurement of extracellular monoamine level based on the intracerebral microdialysis and the intracranial self-stimulation test, and provide biological specimen (animal brains etc.) of animals that have been administered with controlled substances etc. (Kazutaka Ikeda).
  4. Analyze the effects of medications on the applicant's genetically modified animals and/or cultured cells as models for diseases (Atsumi Nitta).

The Application Procedure (Japanese Only)

3. Support for operation analysis using the optical technology

We conduct the following support at the Graduate School of Medicine and the Faculty of Medicine, The University of Tokyo (Haruhiko Bito)

  1. Detect and operate the multi-dimentional optical signal for cell function.
  2. Develop and provide reagents used for detecting light signal (R-CaMP2, a high-affinity red genetically encoded calcium indicator (GECI)) as well as used in optogenetical operating method (ChR2 etc.).
  3. Any other support above related to optical technology.

The Application Procedure (Japanese Only)

4. Support for analyzing the obtained data using multi-functional electrodes

We provide the following technical support related to research resources in neurophysiology at Tohoku University School of Medicine. (Hajime Mushiake)
Please refer to: https://www.lbc.mech.tohoku.ac.jp/physio-sien/

  1. Multiple electrodes for recording activities of several nerve cells.
  2. Organic microelectrodes Organic microelectrodes made of carbon are flexible, biocompatible, and can be used under MRI recording
  3. * Facility for small animals to experience the virtual reality. (Device for a wide-visual display action target and its software)
  4. * Fiber for intracerebral imaging. (Imaging system using micro-fiber to record the data in the deep brain.)
  5. Activity-dependent manganese contrast MRI
  6. support of data analysis acquired by Measurement method (1-5).

*Please be noted the support services above with * mark were limited to those who can afford the charge, who can accept the limited quantity, and who can conduct a collaborative research.

The Application Procedure (Japanese Only)

Contact

Please inquire the detail of our support. Please replace {at} with @ in the email addresses

Support for behavioral analysis Tsuyoshi Miyakawa (cgbb{at}fujita-hu.ac.jp)
Keizo Takao (takao{at}cts.u-toyama.ac.jp)
Support for pharmacological analysis Kazutaka Ikeda (ikeda-kz{at}igakuken.or.jp)
Atsumi Nitta (nitta{at}pha.u-toyama.ac.jp)
Support for operation analysis using the optical technology Haruhiko Bito (opto-shien{at}m.u-tokyo.ac.jp)
Support for analyzing the obtained data using multi-functional electrodes Hajime Mushiake (physio-shien{at}umin.ac.jp)

Note prior to file an application

Term of the research We schedule to conduct our support service from when the applicant is adopted KAKENHI in 2022 to March 2023. We would like to set the support term of each subjects after the selection.
Consent from the applicant’s organization Applicant is required to obtain the consent of the applicant's organization prior to our support service. We need the applicant's sign on the written pledge that the applicant is eligible to obtain the consent of the applicant's affiliated organization.
Intellectual Property Rights The Intellectual Property Rights obtained through the support program will belong to the applicant.
Research Report Please submit two reports to the support group: one is to describe the progress of the applicant's research (deadline: in the end of the fiscal year when the applicant is supported), and the other is to describe the applicant's result when the applicant publish academic papers or when the applicant's patent has been registered.
Bioethics and Biosafety Applicant must conduct the predetermined procedure if the applicant's research category requires approval, report, confirmation, etc. regarding bioethics and biosafety from the director of the applicant's affiliated organization or institution.
If the applicant fails to follow the procedures above, or the applicant fail to be applied to the guide set by the Japanese government (refer to the MEXT's website "Guidelines on Bioethics and Biosafety" http://www.mext.go.jp/a_menu/shinkou/seimei/main.htm), the applicant may be excluded from the selection or cancelled from the selection result.
Attention to the human rights and interest protection If the applicant's project includes research development or investigation that need the consent or cooperation of the relevant parties or the social consensus, please take appropriate measures on the human rights and interest protection prior to the submission.
Management and use of the Applicants’ Information We carefully manage the information we obtained upon application and selection, and we will not make the information public without the applicant's consent.
Acknowledgement Please include the statement upon publication of the applicant’s paper as described below.
< Description of Acknowledgement>
This work was supported by JSPS KAKENHI Grant Number JP 22H04922 (AdAMS).