Support for molecular profiling

Head of the Group
Hiroyuki Seimiya (Cancer Chemotherapy Center of JFCR)

Activity

We will provide support for molecular profiling and screening based on chemical biology and bioinformatics. This support will both create excellent small-molecule bioprobes, and screen functional molecules relating to a vital phenomenon. This support will promote cross-sectional researches ranging from the small molecules to cell level, which will be hardly covered by the support using animal models alone. Through these activities, we contribute to make a breakthrough for life science research in Japan.

I. Support for molecular profiling: elucidate and confirm a biological activity and physiological property of chemical compound.

We evaluate the effects of the applicant’s chemical compounds on cell growth and morphological characteristics, and gene and protein expression. Employing bioinformatics analyses with a comprehensive database, we predict the target molecules of the test compounds. We offer the applicant a considerable advantage if the person is looking for support elucidating the unknown physiological activity of the novel compound that the applicant has isolated or synthesized, or support validating the specificity of a chemical compound as a small-molecule bioprobe and its off-target effects.

Details of our support:

II. Support for molecular screening: identify chemical compounds and genes having impact on a vital phenomenon

We construct and distribute libraries of chemical compounds having defined targets (kinase inhibitors etc.) and small interfering RNAs. These libraries are useful in various purposes, including screening for regulators of biological events of the applicant’s interest and pilot tests of novel screening systems. Also we support to identify the regulator genes in vital phenomenon using functional genomics search with the use of barcode shRNA libraries.

Details of our support:

Construction and distribution of chemical libraries (Kawada)
Construction and distribution of RNA interference kits (Tahara)
Molecular screening with a barcode shRNA sequence technique (Yoshida)
Estimation of protein-protein interactions using Surface Plasmon Resonance（Taniguchi）
Construction of a comprehensive database and in silico analysis (Matsuura and Ushijima)

Members for this support

Name	Affiliation	Role
Hiroyuki Seimiya	Molecular Biotherapy Chief, Cancer Chemotherapy Center of JFCR	General Management for Support for Molecular Profiling and molecular profiling based on transcriptome analysis
Hiroyuki Osada	Group Director, Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science	Molecular profiling based on the proteomic analysis
Shingo Dan	Molecular Pharmacology Chief, Cancer Chemotherapy Center of JFCR	Molecular profiling based on the growth analysis across the cell line panels
Masaya Imoto	Project Professor, Juntendo University Graduate School of Medicine	Molecular profiling based on the cell phenotypic analysis
Etsu Tashiro	Associate Professor, Laboratory of Biochemistry, Showa Pharmaceutical University	Molecular profiling based on the cell phenotypic analysis
Manabu Kawada	Laboratory Head, Laboratory of Oncology, Institute of Microbial Chemistry	Construction and distribution of chemical libraries
Hidetoshi Tahara	Professor, Department of Cellular and Molecular Biology, Institute of Biomedical & Health Sciences, Hiroshima University	Construction and distribution of RNA interference kits
Minoru Yoshida	Group Director, Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Science	Molecular screening with a barcode shRNA sequence technique
Masaaki Matsuura	Professor, Biostatistics, Teikyo University	Construction of a comprehensive database and in silico analysis
Masaru Ushijima	Research Scientist, Cancer Institute Hospital of JFCR	Construction of a comprehensive database and in silico analysis
Research Supporter
Takao Yamori	Professor, Teikyo Academic Research Center (TARC), Teikyo University	Assistant for the general management of molecular profiling group
Akihiro Tomida	Genome Research Chief, Cancer Chemotherapy Center of JFCR	Data analysis on the comprehensive gene expression
Makoto Muroi	Senior Research Scientist, Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science	Multivariate analysis using proteome analysis
Hiroaki Taniguchi	Associate Professor, Clinical and Translational Research Center, Keio University Hospital	Estimation of protein-protein interactions using Surface Plasmon Resonance

Click here for more detail of the support for molecular profiling（http://molpro.jfcr.or.jp）
Contact to cads@jfcr.or.jp about the support for molecular profiling

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